Role of Interaction and Nucleoside Diphosphate Kinase B in Regulation of the Cystic Fibrosis Transmembrane Conductance Regulator Function by cAMP-Dependent Protein Kinase A
Identifieur interne : 001032 ( Main/Exploration ); précédent : 001031; suivant : 001033Role of Interaction and Nucleoside Diphosphate Kinase B in Regulation of the Cystic Fibrosis Transmembrane Conductance Regulator Function by cAMP-Dependent Protein Kinase A
Auteurs : Lee A. Borthwick [Royaume-Uni] ; Mathieu Kerbiriou [Royaume-Uni] ; Christopher J. Taylor [Royaume-Uni] ; Giorgio Cozza [Italie] ; Ioan Lascu [France] ; Edith H. Postel [États-Unis] ; Diane Cassidy [Royaume-Uni] ; Pascal Trouvé [France] ; Anil Mehta [Royaume-Uni] ; Louise Robson [Royaume-Uni] ; Richmond Muimo [Royaume-Uni]Source :
- PLoS ONE [ 1932-6203 ] ; 2016.
Descripteurs français
- KwdFr :
- AMP cyclique (métabolisme), Animaux, Appareil respiratoire (cytologie), Cellules épithéliales (cytologie), Cyclic AMP-Dependent Protein Kinases (métabolisme), Cytosol (métabolisme), Données de séquences moléculaires, Humains, Jeune adulte, Liaison aux protéines, Lignée cellulaire, Membrane cellulaire (métabolisme), Modèles moléculaires, NM23 Nucleoside Diphosphate kinases (), NM23 Nucleoside Diphosphate kinases (métabolisme), Polarité de la cellule, Protéine CFTR (), Protéine CFTR (métabolisme), Sites de fixation, Structure tertiaire des protéines, Séquence d'acides aminés, Transport de protéines.
- MESH :
- cytologie : Appareil respiratoire, Cellules épithéliales.
- métabolisme : AMP cyclique, Cyclic AMP-Dependent Protein Kinases, Cytosol, Membrane cellulaire, NM23 Nucleoside Diphosphate kinases, Protéine CFTR.
- Animaux, Données de séquences moléculaires, Humains, Jeune adulte, Liaison aux protéines, Lignée cellulaire, Modèles moléculaires, NM23 Nucleoside Diphosphate kinases, Polarité de la cellule, Protéine CFTR, Sites de fixation, Structure tertiaire des protéines, Séquence d'acides aminés, Transport de protéines.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Binding Sites, Cell Line, Cell Membrane (metabolism), Cell Polarity, Cyclic AMP (metabolism), Cyclic AMP-Dependent Protein Kinases (metabolism), Cystic Fibrosis Transmembrane Conductance Regulator (chemistry), Cystic Fibrosis Transmembrane Conductance Regulator (metabolism), Cytosol (metabolism), Epithelial Cells (cytology), Humans, Models, Molecular, Molecular Sequence Data, NM23 Nucleoside Diphosphate Kinases (chemistry), NM23 Nucleoside Diphosphate Kinases (metabolism), Protein Binding, Protein Structure, Tertiary, Protein Transport, Respiratory System (cytology), Young Adult.
- MESH :
- chemical , chemistry : Cystic Fibrosis Transmembrane Conductance Regulator, NM23 Nucleoside Diphosphate Kinases.
- chemical , metabolism : Cyclic AMP, Cyclic AMP-Dependent Protein Kinases, Cystic Fibrosis Transmembrane Conductance Regulator, NM23 Nucleoside Diphosphate Kinases.
- cytology : Epithelial Cells, Respiratory System.
- metabolism : Cell Membrane, Cytosol.
- Amino Acid Sequence, Animals, Binding Sites, Cell Line, Cell Polarity, Humans, Models, Molecular, Molecular Sequence Data, Protein Binding, Protein Structure, Tertiary, Protein Transport, Young Adult.
Abstract
Cystic fibrosis results from mutations in the cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-dependent protein kinase A (PKA) and ATP-regulated chloride channel. Here, we demonstrate that nucleoside diphosphate kinase B (NDPK-B, NM23-H2) forms a functional complex with CFTR. In airway epithelia forskolin/IBMX significantly increases NDPK-B co-localisation with CFTR whereas PKA inhibitors attenuate complex formation. Furthermore, an NDPK-B derived peptide (but not its NDPK-A equivalent) disrupts the NDPK-B/CFTR complex
Url:
DOI: 10.1371/journal.pone.0149097
PubMed: 26950439
PubMed Central: 4780765
Affiliations:
- France, Italie, Royaume-Uni, États-Unis
- Aquitaine, New Jersey, Nouvelle-Aquitaine, Région Bretagne
- Bordeaux, Brest
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Le document en format XML
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Postel</name><affiliation wicri:level="2"><nlm:aff id="aff006"><addr-line>Robert Wood Johnson Medical School, UMDNJ, New Brunswick, New Jersey, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Robert Wood Johnson Medical School, UMDNJ, New Brunswick, New Jersey</wicri:regionArea><placeName><region type="state">New Jersey</region></placeName></affiliation></author><author><name sortKey="Cassidy, Diane" sort="Cassidy, Diane" uniqKey="Cassidy D" first="Diane" last="Cassidy">Diane Cassidy</name><affiliation wicri:level="1"><nlm:aff id="aff007"><addr-line>Medical Research Institute/CVS Diabetes Lung, Ninewells Hospital and Medical School, University of Dundee, Dundee, DD1 9SY, United Kingdom</addr-line></nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Medical Research Institute/CVS Diabetes Lung, Ninewells Hospital and Medical School, University of Dundee, Dundee, DD1 9SY</wicri:regionArea><wicri:noRegion>DD1 9SY</wicri:noRegion></affiliation></author><author><name sortKey="Trouve, Pascal" sort="Trouve, Pascal" uniqKey="Trouve P" first="Pascal" last="Trouvé">Pascal Trouvé</name><affiliation wicri:level="3"><nlm:aff id="aff008"><addr-line>Inserm UMR 1078, Génétique, Génomique Fonctionnelle et Biotechnologies, 46 rue Félix Le Dantec, 29218, Brest Cedex2, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Inserm UMR 1078, Génétique, Génomique Fonctionnelle et Biotechnologies, 46 rue Félix Le Dantec, 29218, Brest Cedex2</wicri:regionArea><placeName><region type="region" nuts="2">Région Bretagne</region><settlement type="city">Brest</settlement></placeName></affiliation></author><author><name sortKey="Mehta, Anil" sort="Mehta, Anil" uniqKey="Mehta A" first="Anil" last="Mehta">Anil Mehta</name><affiliation wicri:level="1"><nlm:aff id="aff007"><addr-line>Medical Research Institute/CVS Diabetes Lung, Ninewells Hospital and Medical School, University of Dundee, 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Borthwick</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Academic Unit of Respiratory Medicine, Department of Infection and Immunity, The University of Sheffield, The Medical School, Sheffield, S10 2RX, United Kingdom</addr-line></nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Academic Unit of Respiratory Medicine, Department of Infection and Immunity, The University of Sheffield, The Medical School, Sheffield, S10 2RX</wicri:regionArea><wicri:noRegion>S10 2RX</wicri:noRegion></affiliation></author><author><name sortKey="Kerbiriou, Mathieu" sort="Kerbiriou, Mathieu" uniqKey="Kerbiriou M" first="Mathieu" last="Kerbiriou">Mathieu Kerbiriou</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Academic Unit of Respiratory Medicine, Department of Infection and Immunity, The University of Sheffield, The Medical School, Sheffield, S10 2RX, United Kingdom</addr-line></nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Academic Unit of Respiratory Medicine, Department of Infection and Immunity, The University of Sheffield, The Medical School, Sheffield, S10 2RX</wicri:regionArea><wicri:noRegion>S10 2RX</wicri:noRegion></affiliation></author><author><name sortKey="Taylor, Christopher J" sort="Taylor, Christopher J" uniqKey="Taylor C" first="Christopher J." last="Taylor">Christopher J. Taylor</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Academic Unit of Child Health, University of Sheffield, Stephenson Wing, Sheffield Children's Hospital, Sheffield, S10 2TH, United Kingdom</addr-line></nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Academic Unit of Child Health, University of Sheffield, Stephenson Wing, Sheffield Children's Hospital, Sheffield, S10 2TH</wicri:regionArea><wicri:noRegion>S10 2TH</wicri:noRegion></affiliation></author><author><name sortKey="Cozza, Giorgio" sort="Cozza, Giorgio" uniqKey="Cozza G" first="Giorgio" last="Cozza">Giorgio Cozza</name><affiliation wicri:level="1"><nlm:aff id="aff004"><addr-line>Department of Biomedical Sciences, University of Padova, Via Ugo Bassi 58/B 35131, Padova, Italy</addr-line></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Biomedical Sciences, University of Padova, Via Ugo Bassi 58/B 35131, Padova</wicri:regionArea><wicri:noRegion>Padova</wicri:noRegion></affiliation></author><author><name sortKey="Lascu, Ioan" sort="Lascu, Ioan" uniqKey="Lascu I" first="Ioan" last="Lascu">Ioan Lascu</name><affiliation wicri:level="3"><nlm:aff id="aff005"><addr-line>University of Bordeaux, France, and Institut de Biochimie et Genetique Cellulaires, Centre Nationale de la Recherche Scientifique UMR 5095, 33077, Bordeaux, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>University of Bordeaux, France, and Institut de Biochimie et Genetique Cellulaires, Centre Nationale de la Recherche Scientifique UMR 5095, 33077, Bordeaux</wicri:regionArea><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Bordeaux</settlement></placeName></affiliation></author><author><name sortKey="Postel, Edith H" sort="Postel, Edith H" uniqKey="Postel E" first="Edith H." last="Postel">Edith H. Postel</name><affiliation wicri:level="2"><nlm:aff id="aff006"><addr-line>Robert Wood Johnson Medical School, UMDNJ, New Brunswick, New Jersey, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Robert Wood Johnson Medical School, UMDNJ, New Brunswick, New Jersey</wicri:regionArea><placeName><region type="state">New Jersey</region></placeName></affiliation></author><author><name sortKey="Cassidy, Diane" sort="Cassidy, Diane" uniqKey="Cassidy D" first="Diane" last="Cassidy">Diane Cassidy</name><affiliation wicri:level="1"><nlm:aff id="aff007"><addr-line>Medical Research Institute/CVS Diabetes Lung, Ninewells Hospital and Medical School, University of Dundee, Dundee, DD1 9SY, United Kingdom</addr-line></nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Medical Research Institute/CVS Diabetes Lung, Ninewells Hospital and Medical School, University of Dundee, Dundee, DD1 9SY</wicri:regionArea><wicri:noRegion>DD1 9SY</wicri:noRegion></affiliation></author><author><name sortKey="Trouve, Pascal" sort="Trouve, Pascal" uniqKey="Trouve P" first="Pascal" last="Trouvé">Pascal Trouvé</name><affiliation wicri:level="3"><nlm:aff id="aff008"><addr-line>Inserm UMR 1078, Génétique, Génomique Fonctionnelle et Biotechnologies, 46 rue Félix Le Dantec, 29218, Brest Cedex2, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Inserm UMR 1078, Génétique, Génomique Fonctionnelle et Biotechnologies, 46 rue Félix Le Dantec, 29218, Brest Cedex2</wicri:regionArea><placeName><region type="region" nuts="2">Région Bretagne</region><settlement type="city">Brest</settlement></placeName></affiliation></author><author><name sortKey="Mehta, Anil" sort="Mehta, Anil" uniqKey="Mehta A" first="Anil" last="Mehta">Anil Mehta</name><affiliation wicri:level="1"><nlm:aff id="aff007"><addr-line>Medical Research Institute/CVS Diabetes Lung, Ninewells Hospital and Medical School, University of Dundee, Dundee, DD1 9SY, United Kingdom</addr-line></nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Medical Research Institute/CVS Diabetes Lung, Ninewells Hospital and Medical School, University of Dundee, Dundee, DD1 9SY</wicri:regionArea><wicri:noRegion>DD1 9SY</wicri:noRegion></affiliation></author><author><name sortKey="Robson, Louise" sort="Robson, Louise" uniqKey="Robson L" first="Louise" last="Robson">Louise Robson</name><affiliation wicri:level="1"><nlm:aff id="aff003"><addr-line>Department of Biomedical Science, The University of Sheffield, Western Bank, Sheffield, S10 2TN, United Kingdom</addr-line></nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Biomedical Science, The University of Sheffield, Western Bank, Sheffield, S10 2TN</wicri:regionArea><wicri:noRegion>S10 2TN</wicri:noRegion></affiliation></author><author><name sortKey="Muimo, Richmond" sort="Muimo, Richmond" uniqKey="Muimo R" first="Richmond" last="Muimo">Richmond Muimo</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Academic Unit of Respiratory Medicine, Department of Infection and Immunity, The University of Sheffield, The Medical School, Sheffield, S10 2RX, United Kingdom</addr-line></nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Academic Unit of Respiratory Medicine, Department of Infection and Immunity, The University of Sheffield, The Medical School, Sheffield, S10 2RX</wicri:regionArea><wicri:noRegion>S10 2RX</wicri:noRegion></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Binding Sites</term><term>Cell Line</term><term>Cell Membrane (metabolism)</term><term>Cell Polarity</term><term>Cyclic AMP (metabolism)</term><term>Cyclic AMP-Dependent Protein Kinases (metabolism)</term><term>Cystic Fibrosis Transmembrane Conductance Regulator (chemistry)</term><term>Cystic Fibrosis Transmembrane Conductance Regulator (metabolism)</term><term>Cytosol (metabolism)</term><term>Epithelial Cells (cytology)</term><term>Humans</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>NM23 Nucleoside Diphosphate Kinases (chemistry)</term><term>NM23 Nucleoside Diphosphate Kinases (metabolism)</term><term>Protein Binding</term><term>Protein Structure, Tertiary</term><term>Protein Transport</term><term>Respiratory System (cytology)</term><term>Young Adult</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>AMP cyclique (métabolisme)</term><term>Animaux</term><term>Appareil respiratoire (cytologie)</term><term>Cellules épithéliales (cytologie)</term><term>Cyclic AMP-Dependent Protein Kinases (métabolisme)</term><term>Cytosol (métabolisme)</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Jeune adulte</term><term>Liaison aux protéines</term><term>Lignée cellulaire</term><term>Membrane cellulaire (métabolisme)</term><term>Modèles moléculaires</term><term>NM23 Nucleoside Diphosphate kinases ()</term><term>NM23 Nucleoside Diphosphate kinases (métabolisme)</term><term>Polarité de la cellule</term><term>Protéine CFTR ()</term><term>Protéine CFTR (métabolisme)</term><term>Sites de fixation</term><term>Structure tertiaire des protéines</term><term>Séquence d'acides aminés</term><term>Transport de protéines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Cystic Fibrosis Transmembrane Conductance Regulator</term><term>NM23 Nucleoside Diphosphate Kinases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cyclic AMP</term><term>Cyclic AMP-Dependent Protein Kinases</term><term>Cystic Fibrosis Transmembrane Conductance Regulator</term><term>NM23 Nucleoside Diphosphate Kinases</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Appareil respiratoire</term><term>Cellules épithéliales</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Epithelial Cells</term><term>Respiratory System</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Membrane</term><term>Cytosol</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>AMP cyclique</term><term>Cyclic AMP-Dependent Protein Kinases</term><term>Cytosol</term><term>Membrane cellulaire</term><term>NM23 Nucleoside Diphosphate kinases</term><term>Protéine CFTR</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Binding Sites</term><term>Cell Line</term><term>Cell Polarity</term><term>Humans</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>Protein Binding</term><term>Protein Structure, Tertiary</term><term>Protein Transport</term><term>Young Adult</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Jeune adulte</term><term>Liaison aux protéines</term><term>Lignée cellulaire</term><term>Modèles moléculaires</term><term>NM23 Nucleoside Diphosphate kinases</term><term>Polarité de la cellule</term><term>Protéine CFTR</term><term>Sites de fixation</term><term>Structure tertiaire des protéines</term><term>Séquence d'acides aminés</term><term>Transport de protéines</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Cystic fibrosis results from mutations in the cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-dependent protein kinase A (PKA) and ATP-regulated chloride channel. Here, we demonstrate that nucleoside diphosphate kinase B (NDPK-B, NM23-H2) forms a functional complex with CFTR. In airway epithelia forskolin/IBMX significantly increases NDPK-B co-localisation with CFTR whereas PKA inhibitors attenuate complex formation. Furthermore, an NDPK-B derived peptide (but not its NDPK-A equivalent) disrupts the NDPK-B/CFTR complex <italic>in vitro</italic> (19-mers comprising amino acids 36–54 from NDPK-B or NDPK-A). Overlay (Far-Western) and Surface Plasmon Resonance (SPR) analysis both demonstrate that NDPK-B binds CFTR within its first nucleotide binding domain (NBD1, CFTR amino acids 351–727). Analysis of chloride currents reflective of CFTR or outwardly rectifying chloride channels (ORCC, DIDS-sensitive) showed that the 19-mer NDPK-B peptide (but not its NDPK-A equivalent) reduced both chloride conductances. Additionally, the NDPK-B (but not NDPK-A) peptide also attenuated acetylcholine-induced intestinal short circuit currents. <italic>In silico</italic> analysis of the NBD1/NDPK-B complex reveals an extended interaction surface between the two proteins. This binding zone is also target of the 19-mer NDPK-B peptide, thus confirming its capability to disrupt NDPK-B/CFTR complex. We propose that NDPK-B forms part of the complex that controls chloride currents in epithelia.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Riordan, Jr" uniqKey="Riordan J">JR Riordan</name></author><author><name sortKey="Rommens, Jm" uniqKey="Rommens J">JM Rommens</name></author><author><name sortKey="Kerem, B" uniqKey="Kerem B">B Kerem</name></author><author><name sortKey="Alon, N" uniqKey="Alon N">N Alon</name></author><author><name sortKey="Rozmahel, R" uniqKey="Rozmahel R">R Rozmahel</name></author><author><name sortKey="Grzelczak, Z" uniqKey="Grzelczak Z">Z Grzelczak</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lepple Wienhues, A" uniqKey="Lepple Wienhues A">A Lepple-Wienhues</name></author><author><name sortKey="Wieland, U" uniqKey="Wieland U">U Wieland</name></author><author><name sortKey="Laun, T" uniqKey="Laun T">T Laun</name></author><author><name sortKey="Heil, L" uniqKey="Heil 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Borthwick</name></noRegion><name sortKey="Cassidy, Diane" sort="Cassidy, Diane" uniqKey="Cassidy D" first="Diane" last="Cassidy">Diane Cassidy</name><name sortKey="Kerbiriou, Mathieu" sort="Kerbiriou, Mathieu" uniqKey="Kerbiriou M" first="Mathieu" last="Kerbiriou">Mathieu Kerbiriou</name><name sortKey="Mehta, Anil" sort="Mehta, Anil" uniqKey="Mehta A" first="Anil" last="Mehta">Anil Mehta</name><name sortKey="Muimo, Richmond" sort="Muimo, Richmond" uniqKey="Muimo R" first="Richmond" last="Muimo">Richmond Muimo</name><name sortKey="Robson, Louise" sort="Robson, Louise" uniqKey="Robson L" first="Louise" last="Robson">Louise Robson</name><name sortKey="Taylor, Christopher J" sort="Taylor, Christopher J" uniqKey="Taylor C" first="Christopher J." last="Taylor">Christopher J. Taylor</name></country><country name="Italie"><noRegion><name sortKey="Cozza, Giorgio" sort="Cozza, Giorgio" uniqKey="Cozza G" first="Giorgio" last="Cozza">Giorgio Cozza</name></noRegion></country><country name="France"><region name="Nouvelle-Aquitaine"><name sortKey="Lascu, Ioan" sort="Lascu, Ioan" uniqKey="Lascu I" first="Ioan" last="Lascu">Ioan Lascu</name></region><name sortKey="Trouve, Pascal" sort="Trouve, Pascal" uniqKey="Trouve P" first="Pascal" last="Trouvé">Pascal Trouvé</name></country><country name="États-Unis"><region name="New Jersey"><name sortKey="Postel, Edith H" sort="Postel, Edith H" uniqKey="Postel E" first="Edith H." last="Postel">Edith H. Postel</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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